Document Details
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Document Type |
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Thesis |
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Document Title |
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Antioxidant effect of vitamin E against the toxic effects of malathion insecticide on testicular activity of mice التأثير المضاد للأكسدة لفيتامين هـ ضد التأثيرات السامة للمبيد الحشري ملاثيون على نشاط الخصية في الفئران |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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The present study shed the light on the possible preventive effect of vitamin E against the toxicity of malathion (MP) as one of the organophosphate pesticides commonly used in Saudi Arabia and the study aimed to evaluate its impact on weight of body and testes, the physiological, biochemical parameters, the morphometry of the testes and epididymis as well as the histopathological differences in structure of testes in albino mice. The experiment extended for 30 days and the animals were divided into six groups: control (C), Low dose malathion-treated group (LD), High dose malathion-treated group (HD), vitamin E-treated group (VE), Low dose malathion plus vitamin E-treated group (LD+VE) and High dose malathion plus vitamin E-treated group (HD+VE). The obtained results showed that the treatments with LD and HD have caused highly significant decreases in the average body weight and both right and left testes ratio weights. The results showed that malathion in both doses decreased all hematological indices under study such as the red blood cells (RBCs) count, hemoglobin (HBG) and hematocrit (HCT), the mean corpuscular hemoglobin (MCH) and the mean corpuscular hemoglobin concentration (MCHC) the mean corpuscular volume (MCV),and blood platelets count (PLT) except white blood cells (WBCs) which resulted highly significant increase. In addition, the results of biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatine kinase (CK), glucose (GLU), triglycerides (TG), cholesterol (CHOL) and low density lipoprotein cholesterol (LDL-C) and urea (BUN) were increased after treatment with malathion. On the other hand, total protein (TP), Albumin (ALB) and high density lipoprotein cholesterol (HDL-C) , were decreased. Imbalance in these parameters indicate to deficit in liver and kidney function and disorder in the body generally. High significant increase in the activity of antioxidant enzymes Superoxide Dismutase (SOD) could be due to free radicals .When applying vitamin E with malathion, it was found that VE improved the effect of the pesticide on most parameters examined and the activity of enzymes in mice which illustrates the positive impact of vitamin E as an antioxidant.
Also, light microscopic investigations revealed that malathion exposure was associated with necrosis and edema in the seminiferous tubules and interstitial tissues and laceration of some parts of the tubular walls. Degenerative changes in the seminiferous tubules were also observed in mice that received malathion and supplemented with vitamin E, but milder histopathological changes were observed in the interstitial tissues. Thus, it appears that vitamins E ameliorate malathion testicular toxicity but is not completely protective. |
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Supervisor |
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Dr. Moustafa Hussein Elnaggar |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Co-Supervisor |
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Prof. Osama Abdullah Abuzinadah |
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Added Date |
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Monday, April 3, 2017 |
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Researchers
| سحر جميل مليباري | Melebary, Sahar Jameel | Researcher | Doctorate | |
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